Separation of bound from free hormone in radioimmunoassay of lutropin and follitropin.

Double-label time-resolved immunofluorometry of lutropin and follitropin in serum.

We describe a procedure for the simultaneous immunofluorometric assay of lutropin and follitropin in human serum, based on the use of monoclonal antibodies and of the fluorescent lanthanides Eu3+ and Tb3+. The alpha-chain-specific antibody was used as a common capture antibody on the surfaces of microtitration strips. The anti-beta-follitropin antibody was labeled with Tb3+, the anti-beta-lutro...

Follitropin and lutropin simultaneously measured in urine with a commercial radioimmunoassay kit.

Assessment of the biopotency of follitropin alfa and lutropin alfa combined in one injection: a comparative trial in Sprague-Dawley rats

BACKGROUND The current study was designed to determine if follitropin alfa (recombinant human follicle-stimulating hormone; r-hFSH) and lutropin alfa (recombinant human luteinizing hormone; r-hLH) biopotencies were unchanged by reconstituting in sterile water for injection and mixing prior to injection. METHODS The biopotencies of r-hFSH and r-hLH were determined following injection of female...

Synthetic peptide with inhibin-like activity preferentially inhibits follitropin secretion in comparison with lutropin-releasing hormone antagonists.

Biological activity of a synthetic peptide with inhibin-like activity under in vitro and in vivo conditions was compared with three highly potent synthetic lutropin-releasing hormone antagonists. Unlike the synthetic lutropin-releasing hormone antagonists, which effectively inhibited both lutropin and follitropin secretion from the pituitary, the inhibin-like peptide showed a preferential effec...

Pregnant mare serum gonadotropin. An in vitro biological characterization of the lutropin-follitropin dual activity.

The interaction of highly purified pregnant mare serum gonadotropin (PMSG or eCG) has been studied in rat and equine testis homogenates. In the rat studies, unlabeled PMSG was shown to possess both high levels of intrinsic follitropin-like and lutropin-like activity when the inhibition of radioiodinated follitropin and lutropin binding by unlabeled PMSG was studied. Radioiodinated PMSG exhibite...